Chromatographic Analysis of Naphthoquinones in Plants

Authors

  • P. Babula Department of Natural Drugs, University of Veterinary and Pharmaceutical Sciences,
  • R. Mikelová Department of Chemistry and Biochemistry,
  • V. Adam Department of Chemistry and Biochemistry,
  • D. Potěšil Department of Chemistry and Biochemistry,
  • J. Zehnálek Department of Chemistry and Biochemistry,
  • R. Kizek Department of Chemistry and Biochemistry,
  • L. Havel Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Brno
  • Z. Sladký Department of Natural Drugs, University of Veterinary and Pharmaceutical Sciences,

Abstract

The aim of the work was to optimize the high performance liquid chromatography coupled with a diode array detector for the determination of naphthoquinones (1,4-naphthoquinone, lawsone; juglone and plumbagin). The optimal conditions were: mobile phase 0.1 mol l-1 acetic acid – methanol (35 : 65 v/v), flow rate 0.8 ml min-1, column and detector temperature 40 °C. Detection limits (3 S/N) were 65 ng ml-1, 50 ng ml-1, 75 ng ml-1 and 39 ng ml-1 for lawsone, 1,4-naph¬tho¬qui¬none, juglone and plumbagin, respectively. The optimized method was used for the determination of the mentioned naphthoquinones in plants (Dionaea muscipula, Drosera rotundifolia, Drosera spathulata, Drosera capensis and Paulownia tomentosa).

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Published

2006-05-15

How to Cite

Babula, P., Mikelová, R., Adam, V., Potěšil, D., Zehnálek, J., Kizek, R., Havel, L., & Sladký, Z. (2006). Chromatographic Analysis of Naphthoquinones in Plants. Chemické Listy, 100(4). Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/1937

Issue

Vol. 100 No. 4 (2006)

Section

Articles

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